Genetic Diversity of Wilt and Root Rot Pathogens of Chickpea, as Assessed by RAPD and ISSR

BAYRAKTAR H., DOLAR F. S.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.33, sa.1, ss.1-10, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.3906/tar-0709-37
  • Dergi Adı: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1-10
  • Anahtar Kelimeler: Chickpea, root rot, genetic diversity, RAPD, ISSR, AMPLIFIED POLYMORPHIC DNA, F-SP CICERIS, VEGETATIVE COMPATIBILITY GROUPS, POLYMERASE-CHAIN-REACTION, SIMPLE SEQUENCE REPEAT, FUSARIUM-OXYSPORUM, MOLECULAR CHARACTERIZATION, RANDOM AMPLIFICATION, VARIABILITY, RESISTANCE
  • Ankara Üniversitesi Adresli: Evet

Özet

Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPID (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice's coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. The isolates that were not easily separable using morphological characteristics were classified again according to their branching in dendrograms. A partial correlation was observed between cluster analysis and geographic origin of Fusarium solani isolates. Additionally, high cophenetic correlation values for RAPID and ISSR data, using the MXCOPH algorithm, were observed (0.983 and 0.987, respectively). The results show that both analyses were suitable for the evaluation of genetic polymorphisms among fungal populations.