The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum)

Yildiz M., Ozgen M.

PLANT CELL TISSUE AND ORGAN CULTURE, vol.77, no.1, pp.111-115, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 77 Issue: 1
  • Publication Date: 2004
  • Doi Number: 10.1023/b:ticu.0000016493.03592.c3
  • Journal Name: PLANT CELL TISSUE AND ORGAN CULTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.111-115
  • Keywords: in vitro explant growth, in vitro shoot regeneration, Linum usitatissimum, tissue water content, LEAF, EXPANSION
  • Ankara University Affiliated: No

Abstract

This study was carried out to determine the effect of temporary submersion of hypocotyl segments in water on in vitro explant growth and shoot regeneration on MS ( Murashige and Skoog, 1962) medium supplemented with 1 mg l(-1) BAP (6-benzylaminopurine) and 0.02 mg l(-1) NAA (naphthaleneacetic acid) in three flax cultivars. It was observed that water-treated hypocotyl explants gave rise to the highest values with respect to shoot regeneration percentage, shoot number per hypocotyl, shoot length and total shoot number per Petri dish, successful rooting and plantlet establishment. This procedure may be applicable for other species cultured in vitro.