The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum)

Yildiz M., Ozgen M.

PLANT CELL TISSUE AND ORGAN CULTURE, cilt.77, sa.1, ss.111-115, 2004 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 77 Sayı: 1
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1023/b:ticu.0000016493.03592.c3
  • Dergi Adı: PLANT CELL TISSUE AND ORGAN CULTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.111-115
  • Anahtar Kelimeler: in vitro explant growth, in vitro shoot regeneration, Linum usitatissimum, tissue water content, LEAF, EXPANSION
  • Ankara Üniversitesi Adresli: Hayır

Özet

This study was carried out to determine the effect of temporary submersion of hypocotyl segments in water on in vitro explant growth and shoot regeneration on MS ( Murashige and Skoog, 1962) medium supplemented with 1 mg l(-1) BAP (6-benzylaminopurine) and 0.02 mg l(-1) NAA (naphthaleneacetic acid) in three flax cultivars. It was observed that water-treated hypocotyl explants gave rise to the highest values with respect to shoot regeneration percentage, shoot number per hypocotyl, shoot length and total shoot number per Petri dish, successful rooting and plantlet establishment. This procedure may be applicable for other species cultured in vitro.