Electrochemical, spectroscopic, and molecular docking studies of the interaction between the anti-retroviral drug indinavir and dsDNA

Mollarasouli F., DOĞAN TOPAL B., ÇAĞLAYAN M. G., TAŞKIN TOK T., Ozkan S. A.

Journal of Pharmaceutical Analysis, cilt.10, sa.5, ss.473-481, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jpha.2020.08.004
  • Dergi Adı: Journal of Pharmaceutical Analysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, Directory of Open Access Journals
  • Sayfa Sayıları: ss.473-481
  • Anahtar Kelimeler: Glassy carbon electrode, Calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA), Biosensor, Fluorescence, Molecular docking, GLASSY-CARBON ELECTRODE, ANTICANCER DRUG, PROTEASE INHIBITOR, ANTIVIRAL DRUG, DNA BIOSENSOR, HUMAN PLASMA, NELFINAVIR, NEVIRAPINE, RITONAVIR, LOPINAVIR
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2020 Xi'an Jiaotong UniversityIn this study, an electrochemical DNA biosensor was developed using a straightforward methodology to investigate the interaction of indinavir with calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) for the first time. The decrease in the oxidation signals of deoxyguanosine (dGuo) and deoxyadenosine (dAdo), measured by differential pulse voltammetry, upon incubation with different concentrations of indinavir can be attributed to the binding mode of indinavir to ct-dsDNA. The currents of the dGuo and dAdo peaks decreased linearly with the concentration of indinavir in the range of 1.0–10.0 μg/mL. The limit of detection and limit of quantification for indinavir were 0.29 and 0.98 μg/mL, respectively, based on the dGuo signal, and 0.23 and 0.78 μg/mL, respectively, based on the dAdo signal. To gain further insights into the interaction mechanism between indinavir and ct-dsDNA, spectroscopic measurements and molecular docking simulations were performed. The binding constant (Kb) between indinavir and ct-dsDNA was calculated to be 1.64 × 108 M−1, based on spectrofluorometric measurements. The obtained results can offer insights into the inhibitory activity of indinavir, which could help to broaden its applications. That is, indinavir can be used to inhibit other mechanisms and/or hallmarks of viral diseases.