Genetic Relationships Among Medicago sativa L. Clones Commonly Grown in Central Anatolia

Guelbitti-Onarici S., KENDİR H., SANCAK C., SEVİMAY C. S., Ekiz H., ÖZCAN S.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.15, sa.2, ss.142-147, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1501/tarimbil_0000001084
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.142-147
  • Anahtar Kelimeler: Medicago sativa, amplified fragment length polymorphism, synthetic variety improvement, ALFALFA, DIVERSITY, YIELD, AFLP
  • Ankara Üniversitesi Adresli: Evet

Özet

Amplified fragment length polymorphism (AFLP) markers were used to investigate genotypic variability among 34 alfalfa clones (M. sativa) using 15 primer combinations with restriction enzymes EcoRI, Pstl and Msel. 34 unique AFLP fragments were observed. The 15 primer pairs produced a total of 1002 fragments of which 460 were polymorphic. The number of polymorphic fragments detected per primer combination ranged 7 to 67. Furthermore, 22 clone-specific markers were also detected in the 13 clones. Data analysis was performed with NTSYSpc version 2.1 software. Genetic distance values ranged 5.9374 to 1.1453. Fifteen clones which showed the highest genetic variation were selected for producing synthetic variety of Alfalfa.