Single stage hydrogen production from cellulose through photo-fermentation by a co-culture of Cellulomonas fimi and Rhodopseudomonas palustris

Hitit Z., Lazaro C. Z., Hallenbeck P. C.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.42, no.10, pp.6556-6566, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 10
  • Publication Date: 2017
  • Doi Number: 10.1016/j.ijhydene.2016.12.035
  • Journal Name: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.6556-6566
  • Keywords: Hydrogen yield, COD removal, Substrate concentration, Yeast extract concentration, Microorganism ratio, SWINE WASTE-WATER, CLOSTRIDIUM-ACETOBUTYLICUM X-9, BIOHYDROGEN PRODUCTION, MICROCRYSTALLINE CELLULOSE, RHODOBACTER-CAPSULATUS, DARK FERMENTATION, LIPID PRODUCTION, KEY FACTORS, PHOTOFERMENTATION, THERMOCELLUM
  • Ankara University Affiliated: Yes

Abstract

Biohydrogen production from cellulose by a bacterial co-culture is a potentially promising approach for producing bioenergy from a low cost substrate. The use of a cellulolytic bacterium, Cellulomonas fimi, permits cellulose conversion and the in situ production of substrate for growth and hydrogen production by the photosynthetic bacterium Rhodopseudomonas palustris. Response surface methodology (RSM) with a Box-Behnken design (BBD) was used to examine variations in the key parameters: substrate (cellulose) concentration, yeast extract concentration and the microorganism ratio (Rps. palustris/C. fimi). For the co-culture of R. palustris and C. fimi the highest hydrogen production (44 mmol H-2/L) was achieved at the highest substrate concentration (5 g/L); however, the highest hydrogen yield (3.84 mol H-2/mol glucose equivalent) was observed at the lowest cellulose concentration and highest microorganism ratio. High COD removal efficiencies, over 70%, were achieved over a wide range of conditions and were positively affected by the concentration of yeast extract. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.