In vitro shoot regeneration of fenugreek (Trigonella foenumgraceum L.)


Aasim M., Khawar K. M., SANCAK C., ÖZCAN S.

American-Eurasian Journal of Sustainable Agriculture, cilt.3, sa.2, ss.135-138, 2009 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Sayı: 2
  • Basım Tarihi: 2009
  • Dergi Adı: American-Eurasian Journal of Sustainable Agriculture
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.135-138
  • Anahtar Kelimeler: Fenugreek, IBA, In vitro, Shoot regeneration, TDZ
  • Ankara Üniversitesi Adresli: Evet

Özet

Fenugreek (Trigonella foenumgraceum L.) is legume crop used as medicinal and spice crop throughout the world. Fresh leaves of fenugreek are also used as green vegetable and fodder in some parts of the world; while seeds are used for making pickles in South Asia. The study present efficient shoot regeneration of fenugreek using apical meristem, and cotyledon leaf explants on different concentrations of TDZ with or without IBA. Apical meristem showed higher shoot regeneration potential compared to cotyledon leaf explants. Irrespective of the concentration of plant growth regulators, shoot regeneration was observed on all culture mediums from shoot meris tem explants. Higher number of shoots were recorded on MS medium containing TDZ without IBA compared to the medium containing both TDZ and IBA. However, presence of IBA had positive effect on shoot length. Regenerated shoots could not be rooted on any medium containing IBA. © 2009, American Eurasian Network for Scientific Information.