Akademik Veri Yönetim Sistemi
6th International Eurasian Conference on Biological and Chemical Sciences, 11 - 13 Ekim 2023, ss.1
Abstract
Lung infections caused by
various pathogens are defined as the damage in the lung. Although the severity
of the damage may vary from mild to life threatening, burden of the disease is
considered greater than other diseases which are well-recognized. Since
pathogens causing lung infections, some of which are methicillin-resistant Staphylococcus
aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae, are
becoming resistant to antibiotics worldwide, postbiotics as new and safe
natural compounds that are beneficial to host, have gained attention as an
alternative to antibiotics. The present study aimed to evaluate the
anti-microbial and antioxidant activities of postbiotics derived from Lactobacillus
murinus isolated from the lung microbiota of Spalax spp., which have unique
lung microbiota due to their hypoxic and hypercapnic living conditions. For
this purpose, lung tissue samples aseptically obtained from Spalax sp. were
placed in MRS broth for pre-enrichment. Following pre-enrichment, bacteria were
isolated using pour plate method. Postbiotics were extracted from the isolates
by centrifugation and filtration, ant their anti-microbial activity was tested
against MRSA, P. aeruginosa and K. pneumoniae by agar well
diffusion method. Moreover, antioxidant activity of postbiotics were determined
by investigating total phenolic and flavonoid content, as well as their
effectiveness in eliminating DPPH radical. According to our results,
postbiotics from Lactobacillus murinus displayed strong anti-microbial
activity against all pathogens tested. Inhibition zones were measured as 13 ± 0.4 mm, 11 ± 0.5 mm, 13 ±
0.2 mm for K. pneumonia, MRSA and P. auriginosa, respectively.
Total phenolic content was determined as 7 mg GAE/g while total flavonoid
content was calculated as 6.52 mg/g QE. DPPH radical scavenging activity of
postbiotics was found to be 100% efficient for all concentrations tested. These findings suggest that postbiotics
derived from L. murinis isolated from lung microbiota of Spalax spp. may
be used as an alternative to antibiotics.