Akademik Veri Yönetim Sistemi
Turkish Italian Joint Animal Reproduction Congress, Antalya, Türkiye, 10 - 13 Ekim 2024, ss.53-54, (Özet Bildiri)
Abstract
Introduction
and aim:
The initial successful study on the cryopreservation of drone semen was
conducted using the dimethyl sulfoxide (DMSO) and it is still widely used today
(1). In the
subsequent years, the efficacy of glycerol (GLY), dimethylacetamide (DMA),
ethylene glycol (EG) has been researched lower toxicity than the individual use
of each cryoprotectant (7). Similarly, in other species has (2, 3). Nevertheless,
the desired success has not been achieved due to cytotoxic, genotoxic, and
hyperactivation effects induced by cryoprotectant-related factors, in addition
to cold shock (4-6). A study on the
toxicity of cryoprotectants revealed that the mixture of DMSO and EG exhibited been
demonstrated that the use of cryoprotectant mixtures is more effective (8, 9). In the study,
semen samples of drones were cryopreserved using diluents containing DMSO, DMA,
EG, and GLY alone or in combination with each other. After thawing parameters
such as motility (MOT), plasma membrane integrity (PMI), hypo-osmotic swelling
test (HOST), mitochondrial membrane potential (MMP), and acrosome integrity
(AI) were examined. The study investigated the effectiveness of different
cryoprotectant mixtures in the long-term preservation of drone semen.
Methods: A
total of 100 µl of semen was collected from drones of several colonies in each
trial and mixed. Semen was diluted with a Tris-citrate solution containing 10% DMSO,
EG, DMA, and GLY, and 5% mixtures of each pair, to create groups with 100 ×
10^6/ml spermatozoa in each group. All groups were cryopreserved using a
programmable freezing machine and after thawing were evaluated for
spermatological parameters (M, PMI, HOST, MMP, and AI).
The
differences between the groups were determined using One-Way Analysis of
Variance (ANOVA) and Post-hoc Tukey tests with the help of SPSS v.26
statistical software.
Results: According
to the obtained data, significant differences were found between the groups for
all parameters (P ≤ 0.05). In terms of motility, the most successful groups
were the DMSO and DMSO-EG groups. Regarding PMB, the DMSO, DMSO-EG, and EG-GLY
groups demonstrated success. In terms of HOST, the DMA, DMSO-GLY, and EG-GLY
groups demonstrated superior performance. About MMP, the EG group exhibited the
most significant activity. In terms of AI, the EG, GLY, DMSO-EG, DMA-GLY,
DMA-EG, and GLY-EG groups exhibited the most favorable outcomes.
Discussion
and conclusions: The findings of the study suggest that the utilization
of cryoprotectant mixtures may be a viable alternative to the use of a single
cryoprotectant in the cryopreservation of drone semen. The DMSO-EG and EG-GLY
association in particular gave better results compared to the other groups. It
is thought that this situation arises from the differences in the mechanisms of
action of cryoprotectants (10, 11). It is believed
that the synergistic effect of these two substances is beneficial in the
cryopreservation of drone semen. Similarly, cryopreservation of drone semen
with mixtures of DMSO and trehalose has been shown to improve post-thawing
parameters (12). In conclusion,
the study has demonstrated that different cryoprotectant mixtures have a
positive effect on spermatological parameters in the cryopreservation of drone
semen. However, there is a need to further develop this study with in vivo
experiments.
Keywords: Cryopreservation,
dimethyl sulfoxide, drone semen, ethylene glycol, honey bee