Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes

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Celik M., ÜNAL F., Yuzbasioglu D., Yilmaz S., Aksoy H., Karaman S.

CYTOTECHNOLOGY, cilt.51, sa.2, ss.99-104, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 2
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1007/s10616-006-9015-6
  • Dergi Adı: CYTOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.99-104
  • Anahtar Kelimeler: Thymus kotschyanus var glabrescens extract, mutagenicity, antimutagenicity, sister chromatid exchanges, chromosome aberrations, human lymphocyte culture, MUTAGENICITY, VOLATILES, INDUCTION, CULTURES, TRP-P-2, ASSAY, OILS
  • Ankara Üniversitesi Adresli: Hayır

Özet

In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss, extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10(-5), 10(-4), 10(-3) and 10(-2) mu l ml(-1) concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 mu g ml(-1)) plus 10(-5), 10(-4), 10(-3) and 10(-2) mu l ml(-1) concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10(-2) mu l ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10(-4), 10(-3) and 10(-2) mu l ml(-1) concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.