GREEN SYNTHESIS AND CHARACTERIZATION OF ZINC NANOPARTICLES USING MACLURA POMIFERA (RAFİN.) SCHNEIDER AND THEIR ANTIBACTERIAL, ANTIBIOFILM AND ANTI-QUORUM SENSING ACTIVITIES

Gökdere N., Rızvanoğlu S. S., Karaaslan M., Doğanç F., Ergene B., Eryılmaz M., ...Daha Fazla

14th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Türkiye, 25 - 28 Haziran 2024, ss.1

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1
  • Ankara Üniversitesi Adresli: Evet

Özet

GREEN SYNTHESIS AND CHARACTERIZATION OF ZINC NANOPARTICLES USING MACLURA POMIFERA (RAFİN.) SCHNEIDER AND THEIR ANTIBACTERIAL, ANTIBIOFILM AND ANTI-QUORUM SENSING ACTIVITIES

1,2Gökdere N., 3Rızvanoğlu SS., 4Melek K.,5Doğanç F., 4Burçin E., 3,6Eryılmaz E.,

1Palabıyık İM.

1Ankara University,Department of Analytical Chemistry,Ankara, Türkiye,nutan.gokdere@gmail.com

2Ankara University, Graduate School of Health Sciences, Ankara, TÜRKİYE

3Ankara University, Department of Pharmaceutical Microbiology, Ankara, Türkiye

4Ankara University, Department of Pharmacognosy, Ankara, Türkiye

5Ankara University, Department of Pharmaceutical Chemistry, Ankara, Türkiye

6Acibadem Mehmet Ali Aydinlar University, Department of Pharmaceutical Microbiology, İstanbul,Türkiye

 

Introduction: Maclura pomifera (Rafin.) Schneider is generally known as Osage orange and is rich in phenolic compounds. (Altuner et al. 2012).  Green synthesis methods are non-toxic and cost-effective compared to other methods. (Gour and Jain, 2019). The aim of this study is to synthesize and characterize zinc nanoparticles (ZnNPs) using Maclura pomifera. Then, the antibacterial activity, antibiofilm and anti-quorum sensing (anti-QS) activities of the synthesized nanoparticles were investigated.

 

Material and Method: Ethanolic extract was prepared from Maclura pomifera fruits. The extract was added to the ZnCL2 solution.It was incubated and centrifuged. Characterization studies were performed using UV-Vis, FTIR, XRD, and SEM. For the antibacterial activity, the MIC values of the nanoparticles were determined using the broth microdilution method. The anti-QS activity test was performed by the disc diffusion method.The antibiofilm activity against P. aeruginosa PAO1 was evaluated using the crystal violet assay (Junejo et al., 2023).

 

Results: From the UV-visible spectrum, the peak of ZnNPs was observed to occur at 360nm. SEM analysis showed that the particle size was less than 100nm. The ZnNP exhibited the best antibacterial activity against S. aureus ATCC 25923, with an MIC value of 0,25 mg/ml.In the anti-QS activity test, it was observed that ZnNP had a weak inhibition zone. The percentage biofilm inhibition value of ZnO NP was determined as 46.42%.

 

Conclusions: The characterization results indicate that the nanoparticles were synthesized successfully. Furthermore, ZnNPs appear to have three activities.

 

References

1.     Altuner, E. M., C. Işlek, T. Çeter and H. Alpas (2012). "High hydrostatic pressure extraction of phenolic compounds from Maclura pomifera fruits." African Journal of Biotechnology 11(4): 930-937.

2.     Gour, A., & Jain, N. K. (2019). Advances in green synthesis of nanoparticles. Artificial cells, nanomedicine, and biotechnology, 47(1), 844-851.

3.     Junejo, B., Eryilmaz, M., Rizvanoglu, S.S., Palabiyik, I. M., Ghumro, T., Mallah, A., … & Dragoi, E. N. (2023). Pharmacological assessment of Co3O4, CuO, NiO, and ZnO nanoparticles via antibacterial, anti-biofilm, and anti-quorum sensing activities. Water Science & Technology, 87(11), 2840-2851.