Akademik Veri Yönetim Sistemi
MolBiyoKon'24, İzmir, Türkiye, 12 - 14 Eylül 2024, ss.1
Background / Aim
Breast cancer is considered a significant global health problem, globally. Since conventional treatment strategies cause side effects, novel and effective treatment methods are still required. At this point, the anti-cancer potential of microbial postbiotics attracts wide attention. This study aims to investigate the anti-breast cancer effectiveness of postbiotics derived from Ligilactobacillus animalis.
Materials and Methods
Postbiotics were obtained following the cultivation of the strain. Their selective and effective cytotoxic dose on breast cancer cells (MCF-7) was determined by MTT assay after 48 h co-treatment. Flow cytometry analysis was performed to determine the effects of postbiotics on the apoptosis and cell cycle. The effects of the postbiotics on clonogenic activity, cell motility, and intracellular reactive oxygen species (ROS) accumulation of MCF-7 cells were also evaluated. Furthermore, expression levels of genes related to anti-cancer mechanisms were analyzed by quantitative Real-Time
PCR.
Results
The concentration of 1.500 µg/mL of postbiotics which did not display any cytotoxic effects on the healthy cells but significantly reduced the viability of the MCF-7 cells, was selected for further analysis. Inhibition of cell proliferation was also confirmed by decreased clonogenic activity and cell motility. Moreover, postbiotics induced apoptosis as evidenced by subG1 accumulation, an increase in annexin V, and apoptotic gene activation in MCF-7 cells. Furthermore, postbiotic treatment leads to a significant increase in intracellular ROS levels of MCF-7 cells and an up-regulation of the genes related to endoplasmic reticulum stress-mediated apoptosis.
Conclusion
The results of the current study indicate that postbiotics can be used as an adjuvant approach to cancer treatments.
Anahtar Kelimeler: Postbiotics, Breast Cancer, Apoptosis, Cell Cycle, ER Stress