Akademik Veri Yönetim Sistemi
Applied Ecology and Environmental Research, cilt.17, sa.5, ss.12155-12163, 2019 (SCI-Expanded)
© 2019, ALÖKI Kft., Budapest, Hungary.Molecular genetic information on mollusk species is being increasingly considered for identification and taxonomic status. In this study, we examined the potential of shell material of freshwater mussels from four different locations in Turkey for DNA sequence analysis. A total number of 212 specimens including empty shells were sampled from a river and three lakes. Mitochondrial DNA was extracted from ground shell material with isolation kits and tissue material was also used for reference. Even though the majority of empty shells did not produce high-quality material, DNA extraction from fresh shells had similar results with tissue material. Mitochondrial 28S rDNA partial sequences were analyzed using the neighbour-joining method for genetic divergence. Phylogenetic analysis revealed two well-differentiated clades of Anodonta cygnea and Unio crassus. The results clearly indicated that 28S rRNA sequences obtained from shell material are informative enough to reveal major clades, haplotypes and phylogenetic relationship between four populations. These findings support that quite fresh shell material from freshwater mussels can be a convenient DNA source for molecular studies.