Research Information System
in: Advances in Wheat Breeding, Zencirci,N.,Altay,F.,Baloch,F.S.,Nadeem,M.A.,Ludidi, Editor, Springer-Verlag , Singapore, pp.573-596, 2024
Different methods and techniques have been used to increase production and improve quality in wheat since ancient times. Conventional plant breeding has been at leading edge among the important techniques. The discovery of biotechnological techniques and molecular genetics, such as tissue culture, genetic transformation, and in vitro regeneration, has offered novel possibilities of research to improve signifcant crop species including wheat. Tissue culture studies assist in plant breeding to develop new cultivars with desirable traits through somaclonal variation and in vitro selection. The success of tissue culture depends largely upon the average effect of several important factors including explant tissue, culture medium, plant genotypes, and their interactions. An effcient regeneration system is a prerequisite for the application of genetic transformation study of wheat. Particle bombardment and Agrobacterium-mediated transformation methodologies have been used largely for the introduction of the transgene to wheat.