The development of electrochemical DNA biosensor based on poly-L-methionine and bimetallic AuPt nanoparticles coating: Picomolar detection of Imatinib and Erlotinib

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Eskiköy Bayraktepe D., Yıldız C., Yazan Z.

TALANTA, cilt.257, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 257
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.talanta.2023.124361
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Food Science & Technology Abstracts, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Bimetallic nanoparticles, DNA biosensor, Electropolymerization, Erlotinib, Imatinib, Voltammetry
  • Ankara Üniversitesi Adresli: Evet

Özet

We report on the preparation of a new and simple electrochemical DNA biosensor based on DNA/AuPt/p-L-Met coating on a screen-printed carbon electrode (SPE) and its use in the determination of the cancer therapy agents, Imatinib (IMA) and Erlotinib (ERL). Poly-L-methionine (p-L-Met), gold, and platinum nanoparticles (AuPt) were successfully coated by one-step electrodeposition onto the SPE from a solution containing L-Met, HAuCl4, and H2PtCl6. The immobilization of DNA was achieved by drop-casting on the surface of the modified electrode. Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS), Field-Emission Scanning Electron Microscopy (FE-SEM), Energy-Dispersive X-ray Spectroscopy (EDX), and Atomic Force Microscopy (AFM) were used to investigate the morphology, the structure, and the electrochemical performance of the sensor. Experimental factors influencing the coating and DNA immobilization processes were optimized. The peak currents originating from guanine (G) and adenine (A) oxidation of ds-DNA were used as signals to quantify IMA and ERL in the concentration range 2.33-80 nM and 0.032-1.0 nM with the LODs of 0.18 nM and 0.009 nM, respectively. The biosensor developed was suitable for determining IMA and ERL in human serum and pharmaceutical samples.