Detection of Enterohemolysin and Intimin Genes in Escherichia coli O157:H7 Strains Isolated from Calves and Cattle in Afyonkarahisar - Turkey

KUYUCUOĞLU Y., ŞEKER E., SAREYYÜPOĞLU B., GÜRLER Z.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.17, sa.4, ss.663-666, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 4
  • Basım Tarihi: 2011
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.663-666
  • Anahtar Kelimeler: Calves, Cattle, eaeA, EhlyA, Escherichia coli O157:H7, PCR, TOXIN, ASSOCIATION, CONSUMPTION, HEMOLYSIN, OUTBREAKS, O157H7, EAE
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to detect enterohemolysin (EhlyA) and intimin (eaeA) virulence genes of 14 Escherichia coli O157:H7 strains isolated from 457 fecal samples (237 calves and 220 cattle) by PCR. While EhlyA gene was determined in 13 (92.8%) strains, the eaeA gene was positive in 8 (57.1%) strains. Of the 8 eaeA genes, 4 (50.0%) were obtained from diarrheic calves, 2 (25.0%) from non-diarrheic calves, and 2 (25.0%) from healthy cattle. A total of 7 (50.0%) strains were determined to harbour both of the EhlyA and eaeA genes. This study confirmed that calves, especially diarrheic, and cattle are a reservoir of E. coli O157:H7 strains that may be pathogenic for human.