Akademik Veri Yönetim Sistemi
Spectroscopy Letters, cilt.31, sa.3, ss.633-645, 1998 (SCI-Expanded)
Three new spectrophotometric methods are described for the determination of lisinopril and hydrochlorothiazide in their binary mixture:First derivative spectrophotometry .ratio spectra derivative and Vierorcdt's method. The procedures do not require any prior separation. In the derivative spectrophotometry, the dA/dλ values in the first derivative spectra of the mixture were measured at 269.6 nm for lisinopril and at 279.8 nm for hydrochlorothiazide . The calibration graphs were linear in the range 25.56 - 129.50 μg.ml-1 for lisinopril and 10.60 - 139.80 μg.ml-1 for hydrochlorothiazide . In ratio spectra derivative spectrophotometry, the calibration graphs for 15.66-129.50 μg.ml-1 lisinopril and for 5.93-139.80 μg.ml-1 hydrochlorothiazide were obtained by measunng the signals at 253.7 nm and 243.6 nm for lisinopril and at 280.1 nm and 270.8 nm for hydrochlorothiazide. In Vierordt's method. A1, (1 %, 1 cm) values of lisinopril and hydrochlorothiazide were determined at 259 8 nm and 272.7 nm in the zero - order spectra The quantity of both compounds were calculated by using the A11 (1 %,1 cm) values The methods were successfully applied to a pharmaceutical formulation for determination of both active compounds.