Akademik Veri Yönetim Sistemi
European Wound Mangagement Association 2024, London, İngiltere, 1 - 03 Mayıs 2024, ss.1
Aim: Postbiotics are bioactive substances that are
produced by commensal bacteria during fermentation in the matrix. This
study aims to determine their promising activities important for the wound
healing process.
Method: Postbiotics were obtained from Staphylococcus hominis EIR/HS-1, an isolate of human skin
microbiota, following the
fermentation process. DPPH free radical scavenging assays were used to assess
their antioxidant capacity. The total phenolic and flavonoid compounds were also
determined. Cytotoxic effects and migration ability were evaluated on human
keratinocyte and fibroblast cells. Besides, effects on immune markers and collagen
synthesis were analyzed using ELISA and RT-PCR, respectively. Finally, its metabolites were identified using
chromatographic methods.
Results / Discussion: DPPH radical
scavenging activity was found as 100%. Doses below 1000 µg/mL did not exhibit cytotoxic effects on cells and did not induce
apoptosis. As a result of migration, scratches on cells were closed after 24
hours. Postbiotics did not induce pro-inflammatory cytokine production.
However, they significantly induced collagen synthesis in human fibroblast
cells. Lactic acid, B complex vitamins,
glucose, palmitoleic (C16:1 cis-9) acid, gallocatechin, and epicatechin were
found as the most abundant metabolites.
Conclusion: Our
results suggest that postbiotics obtained from Staphylococcus hominis EIR/HS-1
have potential bioactivities that are important for the wound healing process.
Therefore, postbiotics can be used as a new source for alternative bio-control
strategies.