2.1 GHz electromagnetic field does not change contractility and intracellular Ca2+ transients but decreases beta-adrenergic responsiveness through nitric oxide signaling in rat ventricular myocytes

OLĞAR Y., Hidisoglu E., Celen M. C., Yamasan B. E., Yargicoglu P., ÖZDEMİR S.

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, cilt.91, sa.10, ss.851-857, 2015 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 91 Sayı: 10
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3109/09553002.2015.1068462
  • Dergi Adı: INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.851-857
  • Anahtar Kelimeler: EMF, heart, ventricular myocyte, contractility, calcium, nitric oxide, GATED CALCIUM-CHANNELS, CARDIAC MYOCYTES, 1ST MESSENGER, ACTIVATION, SYNTHASE, EXPOSURE, PHOSPHORYLATION, OVEREXPRESSION, MODULATION, FREQUENCY
  • Ankara Üniversitesi Adresli: Evet

Özet

Purpose: Due to the increasing use of wireless technology in developing countries, particularly mobile phones, the influence of electromagnetic fields (EMF) on biologic systems has become the subject of an intense debate. Therefore, in this study we investigated the effect of 2.1 GHz EMF on contractility and beta-adrenergic (-AR) responsiveness of ventricular myocytes.Materials and methods: Rats were randomized to the following groups: Sham rats (SHAM) and rats exposed to 2.1 GHz EMF for 2 h/day for 10 weeks (EM-10). Sarcomere shortening and Ca2+ transients were recorded in isolated myocytes loaded with Fura2-AM and electrically stimulated at 1 Hz, while L-type Ca2+ currents (I-CaL) were measured using whole-cell patch clamping at 36 1 degrees C. Cardiac nitric oxide (NO) levels were measured in tissue samples using a colorimetric assay kit.Results: Fractional shortening and amplitude of the matched Ca2+ transients were not changed in EM-10 rats. Although the isoproterenol-induced (10(-6) M) I-CaL response was reduced in rats exposed to EMF, basal I-CaL density in myocytes was similar between the two groups (p < 0.01). Moreover, EMF exposure led to a significant increase in nitric oxide levels in rat heart (p < 0.02).Conclusions: Long-term exposure to 2.1 GHz EMF decreases -AR responsiveness of ventricular myocytes through NO signaling.