IN VITRO PLANT REGENERATION FROM HUNGARIAN VETCH (VICIA PANNONICA CRANTZ) USING COTYLEDONARY NODE EXPLANTS

Sahin-Demirbag N., KENDİR H., Khawar K. M., Aasim M.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.22, sa.4, ss.929-932, 2008 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 4
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/13102818.2008.10817581
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.929-932
  • Anahtar Kelimeler: Hungarian Vetch, Vicia pannonica, cotyledonary node, Thidiazuron, indole-3-butyric acid, axillary shoot regeneration, rooting, pulse treatment, SOMATIC EMBRYOGENESIS, NARBONENSIS, THIDIAZURON, CALLUS, L.
  • Ankara Üniversitesi Adresli: Evet

Özet

Hungarian vetch is an important forage plant of Southern Europe and South Western Asia. It is progressively gaining popularity as forage plant among farmers of Turkey. In vitro plantlet regeneration in the plant was achieved from cotyledonary node explants on MS medium containing various concentrations of TDZ The results showed that TDZ concentrations affected frequency of shoot regeneration, mean number of shoots per explants and shoot length in the range of 66.67-100%, 3.28-9.33 and 1.28-3.84 cm respectively. The maximum number of shoots was recorded on MS medium containing 0.45 mg/l TDZ. These were rooted by pulse treatment with 50 mg/l IBA,for 7 min and then culturing on 1/2 concentration of MS micro and macro salts and vitamins oil MS medium containing 0.30, 0.60, 0.90, 1.20 or 1.60 mg/l IBA. Rooting was more successful on non pulse treated shoots. All of them were Put into pots, transferred to growth chamber and then to fields, where they flowered and set seeds.