Agrobacterium-mediated transformation in potato using different explants and co-cultivation media and histochemical detection of pathogenesis-related promoters

Uranbey S., Basalma D., SANCAK C., Er C., Ozcan S.

BIOLOGIA, cilt.60, sa.6, ss.685-690, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 6
  • Basım Tarihi: 2005
  • Dergi Adı: BIOLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.685-690
  • Anahtar Kelimeler: potato, leaf discs, microtuber discs, co-cultivation media, pathogenesis-related promoters, GENETIC-TRANSFORMATION, PLANT TRANSFORMATION, TRANSGENIC TOBACCO, TUMEFACIENS, EXPRESSION, EFFICIENCY, VECTORS, GROWTH
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of the study was to compare different co-cultivation media (solid and liquid) and explants (leaf and microtuber) on Agrobacterium-mediated transformation in potato and to detect the beta-glucuronidase (GUS) expression using plant-originated pathogenesis-related (PR) promoters. Leaf and microtuber discs were inoculated with GV 2260 Agrobacterium p35S GUS-INT, AoPRI-CUS-INT and PR1a-GUS and co-cultivated on either liquid or agar-solidified medium. Liquid co-cultivation medium increased transformation frequency of potato in use of all A. tumefaciens plasmids and explant types. Mean transformation frequency was 15% in liquid medium compared to 4.1% on solid medium for leaf explants. Similarly, liquid medium (3.12%) gave higher transformation frequency than solid medium (1.25%) in microtuber discs. Moreover, low GUS expression in all organs was observed for PR promoters (AoPR1 or PR1a) compared to CaMV35S promoter. Both salicylic acid and wounding treatments enhanced AoPR1 and PR1a promoter gene activity in leaf, stolon, tuber and microtuber.