Akademik Veri Yönetim Sistemi
1303-6025, 2021 (Hakemli Dergi)
In this study was aimed to determine the genotoxic effect of
Ramalina farinacea lichen species against stress sources at the molecular level.
After applying three different heavy metals (Pb2+, Cd2+, and Cr6+) to the R.
farinacea, the extent to which the lichen sample absorbed these metals was
determined by Flame Atomic Absorption Spectroscopy. RAPD and MSAP-AFLP
assays were also used to determine the status of DNA damage. The heavy metal
analysis showed that R. farinacea had high levels of Pb2+, Cd2+, and Cr6+ content.
According to the results obtained from molecular analyses, band changes were
observed against seven primers heavy metal stresses and three primers against UV
stress. An increase in Genomic Template Stability (GTS) was determined during
the time in R. farinacea treated with all heavy metal concentrations. The effect of
UV radiations in R. farinacea revealed the highest polymorphism and the lowest
GTS rate depending on the dose. Among all methylation combinations, Type II
was found to show altered in R. farinacea in response to Pb2+, Cd2+, and Cr6+
contents and UV radiations. R. farinacea can be used at the molecular level as a
biomarker of suitable genotoxic effect. This is the first study to reveal DNA
damage against stress sources using a sample of R. farinacea lichen species.