In vitro bulblet production from immature embryos of ornamental plant Ornithogalum platyphyllum Boiss.

Aryf Y., Sati C., Serkan U., Demyr K. M., Gurbuz B., Nepet A., ...Daha Fazla

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.4, sa.4, ss.25-29, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 4
  • Basım Tarihi: 2009
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.25-29
  • Anahtar Kelimeler: Ornithogalum platyphyllum, bulblet, micropropagation, REGENERATION, TISSUE, LILIACEAE
  • Ankara Üniversitesi Adresli: Evet

Özet

A high frequency bulblet regeneration protocol was developed for ornamental plant Ornithogalum platyphyllum using immature embryos. Immature embryos of O. platyphyllum were cultured on Murashige and Skoog medium (MS) supplemented with different concentrations of N-6-benzylamino-purine (BAP), Kinetin (KIN) and alpha-naphthaleneacetic acid (NAA) with or without pretreatment at 4 degrees C for a week. Large numbers of bulblets (over 14 bulblets/explants) were obtained front without pre-treated immature embryos on a MS medium supplemented with 1 mg l(-1) BAP after 10 months of culture initiation. Pre-treatment of immature embryos at 4 degrees C reduced the bulblet regeneration. Rooted bulblets were transplanted to pots containing potting mixture and grown in a growth chamber and total 175 regenerated plants were acclimatized. High survival ratio (100%) was achieved.