Akademik Veri Yönetim Sistemi
CHROMATOGRAPHIA, cilt.79, sa.19-20, ss.1351-1358, 2016 (SCI-Expanded)
A sensitive, simple, rapid, experimentally convenient, cost-effective, environmentally friendly and high-throughput green chemistry by capillary electrophoresis (CE) approach for the determination of eight polyphenolics frequently found in red wines from USA was carried without using toxic organic modifier. Several parameters which affect the separation were investigated to determine the optimum conditions. At room temperature, the eight polyphenolics could be well separated within 15 min in a 55-cm length capillary at a separation voltage of 26 kV with 40-mM borate buffer (pH 8.9). The method was fully validated showing satisfactory data for all method validation parameters tested. The limits of detection varied from 0.15 to 0.32 mu M. The relative standard deviations of migration varied from 0.208 to 0.630 %. The Californian red wine samples analyzed were bought in the local markets, and the polyphenolic compound recoveries were in the range of 98-99.7 %. The method was successfully applied to the determination of the studied polyphenolics in red wine samples with satisfactory recoveries. Catechin, syringic acid, apigenin, myricetin, luteolin, quercetin, caffeic acid, and gallic acid were detected in all samples, with gallic acid and myricetin occurring in the highest concentration.