Akademik Veri Yönetim Sistemi
LARGE ANIMAL REVIEW, cilt.27, sa.3, ss.115-121, 2021 (SCI-Expanded)
The present study aimed to evaluate the viability of the
embryos following freezing and warming by vitrification after
artificialcollapse in bovine embryos produced in vitro. In vitromaturation,
fertilization, and culture procedures were performed usingoocytes obtained from
ovaries collected from slaughterhouses. Embryonic development was evaluated and
recorded. In total,289 blastocysts were obtained after in vitro production, and
61.94% (179/289) of the obtained blastocysts were graded as CodeI (excellent or
good) quality. Only Code I embryos were used in the study and 60 of these
blastocysts were artificially collapsed(Group 1) and 60 of them used as control
(Group 2). Blastocoelic fluid of the blastocysts from group 1 was aspirated by
enter-ing through trophoblast cells using microinjection pipettes with a
micromanipulator system. Thereafter, blastocysts from bothgroups were vitrified
and warmed with ethylene glycol and glycerol-based protocols and embryonic
development was monitoredfor 24 hours. The post-warm rate of re-expanded
blastocyst was 96.66% (58/60) and 91.66% (55/60) in Group 1 and 2,
respec-tively (P > 0.05). The viability rates at 24 hours were 91.66%
(55/60) and 78.33% (47/60) (P > 0.05), and hatching rates were65% (39/60)
and 11.66% (7/60) (P < 0.05) in Group 1 and 2, respectively. Consequently,
it was found that in vitroproduced blas-tocysts can be vitrified after
artificial collapse and embryo development and viability rates following
warming are quite high.