Akademik Veri Yönetim Sistemi
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.54, sa.1, ss.39-42, 2007 (SCI-Expanded)
In this study, investigation of penetration of some bacterial human pathogens through eggshells of embryonated and market eggs was aimed. In the study, Escherichia coli, Salmonella enteriditis and Campylobacter jejuni strains isolated from chickens were used. Triplicate trials were performed with four groups of 80 eggs, one of which was the control group. Fresh cultures of Escherichia coli, Salmonella enteritidis and Campylobacter jejuni strains were swabbed onto eggshells with sterile swabs. After that, the eggs were incubated at different storage conditions (15 degrees C and 75% relative humidity for embryonated eggs and room conditions for market eggs) and inoculations from eggshells, albumen and egg yolk were performed on 3, 7, 14, 21, 30 days of the trial. Inoculations were made on blood and MacConkey agar for isolation of E. coli and C. jejuni isolations were made on modified Preston agar (CCDA). Salmonella enteritidis was isolated by selective enrichment method and also confirmed by PCR technique. By the help of the isolation studies performed on different days, E. coli and S. enteritidis strains could only be isolated at 3(rd) and 7(th) day after inoculations from eggshells, while no isolation of C jejuni from any of the sites was observed. After the studies performed on days 14, 21, and 30, neither of the inoculated bacteria was isolated. The lack of isolation from albumen and egg yolk showed that these bacteria did not penetrate through the eggshell during the incubation period under the mentioned storage conditions.