Morphological and Anatomical Investigations on in Vitro Micrografts of OHxF 333/Pyrus elaeagrifolia Interstock/Rootstock Combination in Pears

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Dumanoğlu H., Celik A., Büyükkartal H. N., Dousti S.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.20, sa.3, ss.269-279, 2014 (SCI-Expanded, Scopus, TRDizin)

Özet

In this study, possibility of creating a specific interstock / rootstock combination to obtain a clonal, semi dwarf, composite pear rootstock tolerant to various stresses by micrografting was investigated. In vitro shoots of 'Old Home x Farmingdale 333' (Pyrus communis L.) which is a clonal semi dwarf pear rootstock resistant to fireblight and pear decline was used as interstock, and in vitro Pyrus elaeagrifolia Pallas seedlings known as tolerant to Fe-chlorosis, salinity and drought stresses was used as rootstock. Cleft grafting was applied in micrografts. Grafted seedlings were cultured on Murashige and Skoog basal medium with 1/2 strength of macronutrients for 8 weeks under white fluorescent light for 16 h day(-1). Cultures, except the control, received complete darkness either 1 or 2 weeks at the beginning of incubation. Graft take success in the control treatment was significantly higher (97.9%) than darkness treatments of 1 or 2 weeks (90.5% and 82.5%, respectively). Ultrastructural observations with transmission electron microscope revealed that dividing cambial initials reached to 2-3 layers, and new xylem and phloem elements distinctly differentiated in transverse sections of the graft union 8 weeks after micrografting in the control and darkness treatments. The results indicated a successful graft union formation.