Molecular identification and genetic diversity of fusarium species associated with onion fields in Turkey

BAYRAKTAR H., DOLAR F. S.

Journal of Phytopathology, cilt.159, sa.1, ss.28-34, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 159 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1111/j.1439-0434.2010.01715.x
  • Dergi Adı: Journal of Phytopathology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.28-34
  • Anahtar Kelimeler: Fusarium species, Genetic diversity, Onion (Allium cepa L.), PCR detection
  • Ankara Üniversitesi Adresli: Evet

Özet

To identify Fusarium species associated with diseases of root and basal plate of onion, surveys were conducted in seven provinces of Turkey in 2007. Samplings were performed in 223 fields, and 332 isolates belonging to 7 Fusarium spp. were obtained. The isolates were identified as F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. proliferatum, F. redolens, and F. culmorum based on morphological and cultural characteristics. Also, species-specific primers were used to confirm the identity of Fusarium species. F. oxysporum was the most commonly isolated species, comprising 66.57% of the total Fusarium species. F. redolens was identified for the first time in onion-growing areas of Turkey. Selected isolates of each species were evaluated for their aggressiveness on onion plant. F. oxysporum, F. solani, F. acuminatum, F. proliferatum, and F. redolens were highly pathogenic, causing severe damping-off on onion plants cv. Texas Early Grano. Inter-simple sequence repeats (ISSR) markers revealed a high degree of intra- and interspecific polymorphisms among Fusarium spp. © 2010 Blackwell Verlag GmbH.