Akademik Veri Yönetim Sistemi
Journal of Liquid Chromatography and Related Technologies, cilt.28, sa.3, ss.423-437, 2005 (SCI-Expanded)
This work describes a new, fully validated, simple, rapid, selective, and sensitive HPLC method with UV detection for the direct determination of abacavir in pharmaceutical dosage forms, raw materials, spiked human serum, and drug dissolution studies without any time-consuming extraction or evaporation steps prior to drug assay. The mobile phase employed was methanol: acetonitrile: 0.015 M KH2PO4 (36:2.6:61.4 v/v/v) adjusted to pH 6.9 with 5 N NaOH. The samples of 20 μL were injected onto a Waters Spherisorb ODSI (250 × 4.6 mm, 5 μm particle size) column. Ketoprofen was used as internal standard. The flow rate was 1.0 mL min-1. The retention times were 5.49 min for abacavir and 9.15 min for ketoprofen in mobile phase, 5.46 min for abacavir and 9.24 min for ketoprofen in serum samples. The samples were detected at 284 nm. The assay was linear in the concentration range 0.010-20 μmL -1 (r = 0.999) with a slope of 1.35 × 10-3; intercept of 0.0841 and the limit of detection was 0.00093 μg mL-1 in mobile phase and 0.025-20 μg mL-1 (r = 0.999) with a slope of 1.44 × 10-3; intercept of 0.0733 and the limit of detection was 0.00418 μg mL-1 in human serum. The linearity of the detector response for abacavir was determined by plotting peak area ratios vs. concentration. It was successfully applied to the analysis of abacavir pharmaceutical preparations, and human serum samples without any interference by the excipients and endogenous substances. Moreover, the method can be used for the determination of abacavir for monitoring its concentration for in vitro dissolution studies. Copyright © Taylor & Francis, Inc.