Electromembrane extraction as a new approach for determination of free concentration of phenytoin in plasma using capillary electrophoresis
DARU, Journal of Pharmaceutical Sciences, cilt.28, sa.2, ss.615-624, 2020 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 2
- Basım Tarihi: 2020
- Doi Numarası: 10.1007/s40199-020-00366-5
- Dergi Adı: DARU, Journal of Pharmaceutical Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, CINAHL, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
- Sayfa Sayıları: ss.615-624
- Anahtar Kelimeler: Antiepileptic, Microextraction, Plasma protein binding, Unbound fraction, DRUG-PROTEIN BINDING, EQUILIBRIUM DIALYSIS, BASIC DRUGS, CHROMATOGRAPHIC ASSAY, UNBOUND PHENYTOIN, ULTRAFILTRATION, MICROEXTRACTION, LAMOTRIGINE, DIPHENYLHYDANTOIN, CARBAMAZEPINE
- Ankara Üniversitesi Adresli: Evet
Özet
© 2020, Springer Nature Switzerland AG.Abstract: Purpose: Electromembrane extraction is a new membrane-based extraction method in which charged compounds are extracted by an electric field. So far, this method has been used to extract and isolate a variety of acidic and basic drugs from various samples, including blood and plasma. However, in this procedure, it is not yet clear whether only unbound fraction of a drug is extracted or the total drug. The aim of this study is to reveal the nature of drug extraction in the presence of plasma proteins. Methods: To determine the nature of the extraction, the electromembrane extraction was performed from plasma solutions of phenytoin with concentrations 0.03 and 1.0 μg/mL, then the result was compared with the values obtained from the electromembrane extraction of ultrafiltrate of the same solutions (free concentration) and protein-free ultrafiltrate of plasma with final concentration of 0.03 and 1.0 μg/mL (total concentration). For this purpose, EME followed by capillary electrophoresis coupled with diode array detection was optimized and validated. Results: The results showed that the electromembrane extraction method was only able to extract the unbound fraction of phenytoin from plasma samples. The method was validated over a concentration range of 0.03–4 μg/mL. The inter and intra-assay precisions were less than 6.7%. The phenytoin protein binding was also determined to be in agreement with the literature data and confirms the validity of this method. Conclusion: This sensitive and quick EME approach for determining the free concentration of a phenytoin, can be a good alternative to classic methods for therapeutic drug monitoring and pharmacokinetic studies. Graphical abstract: [Figure not available: see fulltext.]