Differential expression of selected histone modifier genes in human solid cancers

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ÖZDAĞ SEVGİLİ H., Teschendorff A. E., Ahmed A. A., Hyland S. J., Blenkiron C., Bobrow L., ...Daha Fazla

BMC GENOMICS, cilt.7, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1186/1471-2164-7-90
  • Dergi Adı: BMC GENOMICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Ankara Üniversitesi Adresli: Evet

Özet

Background: Post-translational modification of histones resulting in chromatin remodelling plays a key role in the regulation of gene expression. Here we report characteristic patterns of expression of 12 members of 3 classes of chromatin modifier genes in 6 different cancer types: histone acetyltransferases ( HATs)- EP300, CREBBP, and PCAF; histone deacetylases ( HDACs)- HDAC1, HDAC2, HDAC4, HDAC5, HDAC7A, and SIRT1; and histone methyltransferases ( HMTs)- SUV39H1 and SUV39H2. Expression of each gene in 225 samples ( 135 primary tumours, 47 cancer cell lines, and 43 normal tissues) was analysed by QRT-PCR, normalized with 8 housekeeping genes, and given as a ratio by comparison with a universal reference RNA.