Differential expression of selected histone modifier genes in human solid cancers
BMC GENOMICS, vol.7, 2006 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 7
- Publication Date: 2006
- Doi Number: 10.1186/1471-2164-7-90
- Journal Name: BMC GENOMICS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Open Archive Collection: AVESIS Open Access Collection
- Ankara University Affiliated: Yes
Abstract
Background: Post-translational modification of histones resulting in chromatin remodelling plays a key role in the regulation of gene expression. Here we report characteristic patterns of expression of 12 members of 3 classes of chromatin modifier genes in 6 different cancer types: histone acetyltransferases ( HATs)- EP300, CREBBP, and PCAF; histone deacetylases ( HDACs)- HDAC1, HDAC2, HDAC4, HDAC5, HDAC7A, and SIRT1; and histone methyltransferases ( HMTs)- SUV39H1 and SUV39H2. Expression of each gene in 225 samples ( 135 primary tumours, 47 cancer cell lines, and 43 normal tissues) was analysed by QRT-PCR, normalized with 8 housekeeping genes, and given as a ratio by comparison with a universal reference RNA.