Akademik Veri Yönetim Sistemi
International Scientific Conference on Cultured Meat, Maastricht, Hollanda, 17 - 19 Kasım 2024, ss.1
Production of cultivated meat using animal muscle
cells grown in bioreactors is a promising technology for the sustainable meat
industry. Although cultivated meat has garnered significant attention, there
are still many hurdles to overcome including cost reduction. This study aims to
investigate the effect of the BIFTEK growth medium supplement which contains
microbiota-derived postbiotics and low concentrations of growth factors (40
ng/mL Insulin Growth Factor-1 and 20 ng/mL Fibroblast Growth Factor-2), on the
growth of bovine muscle satellite cells (BSCs). For this purpose, postbiotics were
extracted from Enterococcus hirae, an isolate from cow milk microbiota,
and bovine satellite cells (bSCs) were isolated from muscle biopsies, and then
identified by flow cytometry and immunofluorescence assay. Following the 5-day co-incubation
of BSCs with BIFTEK growth medium supplement; MTT assay, live/dead staining,
and ROS analysis were performed. According to the results, low concentrations (%1)
of Fetal Bovine Serum (FBS) combined with our growth medium supplement BİFTEK
significantly increased BSC proliferation, similar to 10% FBS, and decreased
intracellular ROS accumulation, compared to the control groups. Fluorescence
microscopy analysis also showed that BSCs in the BIFTEK growth medium
supplement can survive and maintain their morphology. In conclusion, our study showed that the
potential of some molecules in postbiotics with proven antioxidant activities
can preserve the viability of BSCs, which can be useful in this regard but
require further investigation. To the best of our knowledge, this study is the
first attempt to use the postbiotics on cultivated meat technology.