Effect of PDYN rs2281285, rs2235749 and rs910080 gene polymorphisms on the intensity of depression symptoms and negative craving in heroin addicts

AKYÜZLÜ D., Ozkan-Kotiloglu S., YALÇIN ŞAHİNER Ş., Ayaz N.

ARCHIVES OF BIOLOGICAL SCIENCES, cilt.73, sa.4, ss.473-482, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.2298/abs210929041k
  • Dergi Adı: ARCHIVES OF BIOLOGICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.473-482
  • Anahtar Kelimeler: heroin dependence, dynorphins, PDYN variation, depression, negative craving, ASSOCIATION, PRODYNORPHIN, DEPENDENCE, STRESS, SYSTEM, DYNORPHIN, ALCOHOL, SHESIS
  • Ankara Üniversitesi Adresli: Evet

Özet

The present study was undertaken to explore whether prodynorphin (PDYN) polymorphisms have an effect on the intensity of depressive symptoms and negative craving in heroin addicts in a sample of 100 heroin addicts and 108 controls. PDYN rs2281285, rs2225749 and rs910080 polymorphisms were analyzed by PCR-RFLP. Craving and the intensity of depressive symptoms were measured by the Substance Craving Scale and the Beck Depression Inventory-II, respectively. A significant association between depression severity and PDYN rs2281285 (P=0.026) and rs2225749 (P=0.038) polymorphisms was detected. PDYN rs2225749 variation showed a trend association with increased negative craving (P=0.066). We also examined the associations between heroin dependence and PDYN rs2281285, rs2225749 and rs910080 gene polymorphisms at the gene and haplotype levels. The AAA haplotype was more frequent in heroin addicts and shown to be significantly associated with increased risk for heroin dependence (OR, 8.922; 95% CI, 1.116-71.313; P<0.05). PDYN rs2281285 and rs2225749 variations affected the intensity of depressive symptoms, and PDYN rs2225749 polymorphism may contribute to the induction of negative craving in heroin addicts. Haplotype analysis revealed for the first time that addicts with the AAA haplotype of PDYN gene may be more prone to heroin dependence.