HPLC analysis of oleuropein in Olea europaea L

Altinyay Ç., ALTUN M. L.

Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.35, sa.1, ss.1-11, 2006 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 1
  • Basım Tarihi: 2006
  • Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.1-11
  • Anahtar Kelimeler: Branches, Cultivars, HPLC, Leaves, O.europaea, Oleaceae, Oleuropein
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study a simple and sensitive HPLC method for separation and quantitative determination of oleuropein in the leaves and the branches of the natural (Olea europaea L. var. sylvestris) and cultivated (Olea europaea L. var. europaea) varieties of Olea europaea L. has been developed. Oleuropein was determined on a reversed phase C18 column , by using water-acetonitrile-formic acid (84.6:15:0.4) (v/v/v) as a mobile phase . The oleuropein content of cultivated O.europaea (var. europaea) (Bahkesir-Edremit samples) was found to be 3.506% for leaves and 1.438% for branches. For natural O.europaea (var. sylvestris) (Osmaniye samples) , these values were found as 5.197% and 1.462%, respectively. The oleuropein content of cultivated O.europaea (var. europaea) (Konya samples) was determined as 4.020% for leaves and 1.097% for branches.