iPBS retrotransposons ' A Universal Retrotransposons' now in molecular phylogeny of fungal pathogens

ÖZER G., BAYRAKTAR H., BALOCH F. S.

BIOCHEMICAL SYSTEMATICS AND ECOLOGY, cilt.68, ss.142-147, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.bse.2016.07.006
  • Dergi Adı: BIOCHEMICAL SYSTEMATICS AND ECOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.142-147
  • Anahtar Kelimeler: iPBS, Retrotransposons, Fungi, Genetic diversity, GENETIC DIVERSITY, ALTERNARIA, RAPD
  • Ankara Üniversitesi Adresli: Evet

Özet

Methods for the diagnostics and detection of plant pathogens need to be quick, simple, reliable, and cost effective. In the present study, inter-priming binding sites (iPBS)-retrotransposons were used to study the genetic diversity of 96 isolates belonging to 16 different fungal species at intra- and inter-species levels. The 6 iPBS-retrotransposon primers amplified 389 bands, of which 387 were polymorphic (99%) with an average of 64.83 polymorphic fragments per primer. Polymorphism information contents per primers ranged from 0.976 to 0.870 with the average being 0.972. Neighbor-joining analysis differentiated all fungal isolates according to their species, and at the intra-specific level revealed considerable genetic variability among fungal isolates. The data supports the use of iPBS-retrotransposons as 'a universal marker' for the molecular characterization of different fungal species. It also suggests that only one or a few iPBS-retrotransposon primers would be sufficient for the discrimination of fungal isolates at both intra- and inter-species levels. (C) 2016 Elsevier Ltd. All rights reserved.