Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.219, 2025 (SCI-Expanded, Scopus)
In response to the growing demand for environmentally responsible analytical methods, a sensitive electrochemical platform was developed for the trace-level determination of Ritlecitinib (RIT) using a poly (3,4-ethylenedioxythiophene): poly (styrene sulfonate)-modified carbon paste electrode (PEDOT:PSS/CPE). The nanostructured conducting polymer enhanced electron transfer kinetics and active surface area, yielding a broad linear range (0.5–14.0 μM; R2 = 0.998) and ultralow detection and quantification limits (0.0017 and 0.0058 μM, respectively). Recovery experiments in human serum, urine, and pharmaceutical tablets (95.33–101.99 %, RSD < 3 %) confirmed the method's precision, reproducibility, and applicability to complex matrices. A complementary UV-spectrophotometric method at 276 nm was also established, providing a rapid, cost-effective, and cross-validating alternative. This method exhibited a linear range of 10–40 μM with a detection limit of 0.43 μM, intra-day repeatability (RSD = 0.87 %), inter-day repeatability over 5 days (RSD = 1.1 %), and recovery from pharmaceutical samples in the range of 100.3–101.1 %, confirming its accuracy and robustness. All stages of analysis, sampling, sample preparation, and instrumental measurement were optimized to minimize sample size, solvent/reagent use, hazardous waste, and energy demand, thereby aligning with green analytical chemistry principles. The environmental friendliness and sustainability of the developed methods were evaluated using three complementary tools: GAPI, AGREE, and BAGI, confirming their minimal ecological impact. Furthermore, this dual-technique platform offers a robust, sensitive, and environmentally responsible solution for pharmaceutical quality control, pharmacokinetic monitoring, and clinical diagnostics.