DESCRIPTION AND MOLECULAR CHARACTERIZATION OF RICHTERSIUS ZIEMOWITI SP. NOV. (RICHTERSIIDAE) FROM NEPAL (ASIA) WITH EVIDENCE OF HETEROZYGOUS POINT MUTATION EVENTS IN THE 28S rRNA

Kayastha P., BERDİ D., Mioduchowska M., Gawlak M., Lukasiewicz A., Goldyn B., ...Daha Fazla

ANNALES ZOOLOGICI, cilt.70, sa.3, ss.381-396, 2020 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.3161/00034541anz2020.70.3.010
  • Dergi Adı: ANNALES ZOOLOGICI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.381-396
  • Anahtar Kelimeler: DNA barcoding, Eutardigrada, heterozygosity, systematics, Tardigrada, taxonomy, water bears, MULTIPLE SEQUENCE ALIGNMENT, HUFELANDI GROUP TARDIGRADA, INTEGRATIVE DESCRIPTION, CONCERTED EVOLUTION, MILNESIUM DOYERE, DNA, EUTARDIGRADA, GENUS, PHYLOGENY, MACROBIOTIDAE
  • Ankara Üniversitesi Adresli: Evet

Özet

Applying an integrative taxonomy approach, we describe a new tardigrade species from Nepal. Light and scanning electron microscopy are used for morphological as well as morphometric analysis of the new species. Furthermore, multilocus molecular data (nuclear markers, i.e., 18S rRNA, 288 rRNA, ITS-2 and mitochondrial COI barcode sequences) were analysed to confirm the novelty of species. The p-distances enabled an independent verification of Richterrsius ziemowiti sp. nov. as species new to science and molecular data allowed us to determine the phylogenetic position of the new species. Morphologically, Richtersius ziemowiti sp. nov. differs from nominal Ric. coronifer mainly by lower pores density on cuticle, smaller eggs and lower number of processes on the egg circumference. What is more, we identified double (overlapping) peaks in mutated loci in the 28S rRNA chromatograms and obtained heterozygous sequences.