Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks

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Ozgur Baydin M., BİLGE DAĞALP S.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.64, no.4, pp.329-335, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.1501/vetfak_0000002817
  • Journal Name: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.329-335
  • Keywords: Bovine herpesvirus 1, caprine herpesvirus 1, gB/gE ELISA, goat, virus neutralisation test, BOVINE HERPESVIRUS-1, CAPRINE-HERPESVIRUS, RUMINANT ALPHAHERPESVIRUSES, SEROLOGICAL EVIDENCE, GLYCOPROTEIN-E, ANTIBODIES, CATTLE, CELLS
  • Ankara University Affiliated: Yes

Abstract

In this study, we have investigated the seroprevalence of alphaherpesvirus (BoHV-1 and CpHV-1) infections in selected goat flocks using glycoprotein B (gB)/glycoprotein E (gE) ELISA combination and virus neutralisation test (VNT). For this purpose, we collected blood serum samples of 546 Saanen goats from Bolu province and tested them by ELISA and VNT. Using ELISA, 32.05% (175/546) of the samples were found to be gB(+)/gE(-) for CpHV-1, whereas only 0.73% (4/546) of the samples were noted to be gB(+)/gE(+) for BoHV-1 releated infection. By performing VNT, we found 31.86% (174/546) and 3.29% (18/546) positivity for CpHV-1 and BoHV-1, respectively. In conclusion, prevalence of CpHV-1 infection was found to be higher than BoHV-1 infection in sampled goat flocks. For diagnosis of alphaherpesviruses in the goats, use of gB blocking ELISA test was found to be favorable as an alternative method. On the other hand, to distinguish between the CpHV-1/BoHV-1, gE blocking ELISA in combination by VNT was found to be incompatible with the statistical analysis (p<0.001). Considering antigenic cross-reactions between these viruses, because of the incompatibility of these tests, using a more sensitive/specific method to determine CpHV-1 and BoHV-1 antibodies can be suggested.