Akademik Veri Yönetim Sistemi
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, cilt.136, 2025 (SCI-Expanded, Scopus)
Barley stripe disease, caused by the fungal pathogen Pyrenophora graminea, is an economically important disease of barley globally. In 2021, 2022, and 2023, 116 P. graminea isolates were collected from various provinces of T & uuml;rkiye. The mating type distribution and prevalence of the isolates were determined. Among the 116 isolates, 89 were identified as MAT-1, while 27 were MAT-2 (chi 2 (1:1) = 33.138 (P <= 0.001)). The ratio of mating types suggests that the Turkish P. graminea population may mainly reproduce asexually. However, in Siirt province, evidence of sexual recombination was found to support sexual reproduction (chi 2 (1:1) = 0.222 (P <= 0.6375)). Most Siirt isolates were obtained from wild barley ( Hordeum spontaneum). To determine the genetic diversity of P. graminea isolates, 3 isolates (2 from Hordeum vulgare and 1 from H. spontaneum) from different regions of T & uuml;rkiye were tested with 75 ISSR primers and 45 iPBS markers. Polymorphic ISSR markers UBC 856, BDB(ACA)5, DDB(CCA)5, UBC 895, UBC 868, UBC 847, and UBC 822, and iPBS markers 2076, 2080, 2078, 2081, and 2399 were identified. UBC 856, UBC 868, 2080, 2081, and 2078 showed the highest number of polymorphic bands. Genetic variation was analyzed using ISRR markers, iPBS markers, and a combination of both markers, resulting in three dendrograms. Dendrograms were separated with a similarity index of 0.65 according to the ISSR marker, 0.55 according to the iPBS marker, and 0.61 in the dendrogram created using both markers. No clear distinction was observed in the isolates based on mating type, virulence value, or geographical origin. Most Ankara isolates in all three dendrograms showed high similarity, suggesting a common gene pool possibly due to infected seed trade and spore dispersion. However, the diverse clustering of isolates from wild barley and the indication of a population structure supporting sexual reproduction in mating types could lead to increased future variation. Monitoring the evolutionary potential of pathogens is important in developing control strategies and gaining insight into their future evolutionary potential.