Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.48, sa.6, ss.911-926, 2024 (SCI-Expanded)
Drought represents a major global threat to the environment, thus leading to food insecurity. Transcription factors (TFs) have a significant role in regulating the expression of stress-related genes. Three-amino acid loop extension (TALE) genes, a type of TF, are known to regulate important biological processes like the maintenance of shoot apical meristems and root formation in plants. In this study, 35 TALE genes were identified and categorized into KNOX-like homeodomain (KNOX) and BLH/BELL-like subfamilies in Phaseolus vulgaris according to the phylogenetic analysis. The detailed characterization of the PvTALE genes using in silico analyses further showed that segmental duplications predominate in the evolution of TALE subfamilies and greatly contribute to the largescale amplification of the gene family. Gene duplications, syntenic relationships, and the nonsynonymous/synonymous substitution ratios suggest intense purifying selection pressure during the evolution of PvTALEs. Furthermore, it was discovered that PvTALEs are regulated by a number of miRNAs and cis-elements, which play important roles in stress response signaling pathways in plants. The PvTALE genes showed tissue specific expression patterns and were differentially expressed in response to drought stress. Proline levels were measured in different tissues of P. vulgaris and were found to be accumulated under drought stress. Based on the obtained findings, it could be concluded that PvTALE genes may contribute to plant growth and drought stress resistance. The basis of the roles and resistance mechanisms of the PvTALE gene family were clarified with this first complete genome-wide study of its kind in P. vulgaris.