Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger

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Uranbey S.

BIOLOGIA PLANTARUM, cilt.49, sa.3, ss.427-430, 2005 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 3
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1007/s10535-005-0021-x
  • Dergi Adı: BIOLOGIA PLANTARUM
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.427-430
  • Anahtar Kelimeler: N-6-benzylaminopurine, henbane, indole-3-butyric acid, alpha-naphthaleneacetic acid, rooting, tissue culture, HIGH-FREQUENCY INDUCTION, PLANT-REGENERATION, EMBRYOGENESIS
  • Ankara Üniversitesi Adresli: Hayır

Özet

A high frequency adventitious shoot regeneration protocol was developed for henbane (Hyoscyamus niger L.) using thidiazuron (TDZ). Hypocotyl, cotyledon and stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of N-6-benzylaminopurine and TDZ. MS medium supplemented with 16 mu M TDZ was the most effective for providing 100 % regeneration frequency associated with a 19.53 shoots per hypocotyl explant. Plantlets were rooted on MS medium supplemented with different concentrations of indole-3-butyric acid (IBA) and alpha-naphthaleneacetic acid. High rooting and survival was achieved using half strength MS medium supplemented with 8 mu M IBA.