Reprogramming of Human Melanocytes and Melanoma Cells with Yamanaka Factors

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Taheri H., Cagin U., YILMAZER AKTUNA A.

PRE-CLINICAL MODELS, cilt.1916, ss.249-261, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1916
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/978-1-4939-8994-2_24
  • Dergi Adı: PRE-CLINICAL MODELS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.249-261
  • Anahtar Kelimeler: Cell reprogramming, iPS cells, Yamanaka factors, Cancer, Disease modelling, Therapy, PLURIPOTENT STEM-CELLS, FIBROBLASTS, EPIGENETICS, INDUCTION, DISEASE
  • Ankara Üniversitesi Adresli: Evet

Özet

The expression of Yamanaka factors (Oct3/4, Klf-4, Sox-2, c-Myc) can reprogram cancer cells to a pluripotent stage. This may cause the removal of their epigenetic memory and result in altered tumorigenicity. Various studies in the literature have shown that cancer cell reprogramming is a potential tool to study disease progression or discover novel therapeutic or diagnostic markers in cancer research. In this chapter, we aim to introduce the cancer cell reprogramming protocol in detail by using human melanocytes and melanoma cell lines, and Sendai viral vectors encoding Yamanaka factors have been used to reprogram cells. Representative results are discussed and important notes have been summarized in order to point out important steps during cancer cell reprogramming.