Akademik Veri Yönetim Sistemi
Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.38, sa.4, ss.257-268, 2009 (Scopus)
The objective of this study was to prepare poly (DL-lactide-co-glycolide) (PLGA) nanoparticles by solvent evaporation method and clarify the effect of various stabilizing agents on the properties of nanoparticles. It is essential to use stabilizing agents to obtain stable emulsions during the nanoparticle preparation process. The type and ratio of the stabilizing agents are very effective on the particle size of the final product. Poly(vinyl alcohol) (PVA) and Pluronic polymers are the commonly used stabilizing agents in PLGA nanoparticle preparation. In this study, a series of nanoparticle formulations containing PVA and/or several Pluronic polymers such as Pluronic F127, Pluronic P85, Pluronic F68, and Pluronic L64 as stabilizing agents were prepared. The influence of PVA concentration and Pluronic polymers on the size, shape and zeta potential of the nanoparticles were evaluated. The size and zeta potential of the nanoparticles were significantly affected by the concentration of PVA. Upon Pluronic introduction the monodisperse size distribution of nanoparticles with PVA was changed into polydisperse distributions. The Field Emission Scanning Electron Microscopy (FESEM) showed that nanoparticles were spherical in shape. The surface of the particles were rough due to Pluronic addition. This research showed that the choice of stabilizing agent is a very critical parameter in terms of obtaining nanoparticles with desired properties. Further studies are being planned to evaluate the efficacy of the prepared formulations on carrying problematic hydrophobic active agents.