HPLC-MS analysis of ipecacuanha alkaloids in pharmaceutical relics from eighteenth century

Nesmerak K., Kudlacek K., Sticha M., Cerveny V., Kunesova J., YILDIZ İ.

MONATSHEFTE FUR CHEMIE, vol.149, no.9, pp.1535-1542, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 149 Issue: 9
  • Publication Date: 2018
  • Doi Number: 10.1007/s00706-018-2223-x
  • Journal Name: MONATSHEFTE FUR CHEMIE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1535-1542
  • Keywords: High-performance liquid chromatography, Decomposition, Long-term stability, Mass spectrometry, Emetine, Cephaeline, PERFORMANCE LIQUID-CHROMATOGRAPHY, EMETINE, CEPHAELINE, BIOSYNTHESIS, SYRUP, ASSAY, ROOT
  • Ankara University Affiliated: Yes

Abstract

The alkaloids in two historical pharmaceutical relics of ipecacuanha, dated to the eighteenth century, were analyzed by HPLC-MS. The conditions of extraction and separation were optimized. Four alkaloids (emetine, cephaeline, protoemetine, and O-methylpsychotrine) were found. The content of the main ipecacuanha alkaloids, emetine and cephaeline, in analyzed samples is fairly decreased in comparison with standard current pharmaceutical substance. O-Methylpsychotrine has been identified as the emetine decomposition product. The identity of the compounds was confirmed by MS2, and the ESI+ fragmentation mechanisms of compounds found have been proposed.