HPLC-MS analysis of ipecacuanha alkaloids in pharmaceutical relics from eighteenth century

Nesmerak K., Kudlacek K., Sticha M., Cerveny V., Kunesova J., YILDIZ İ.

MONATSHEFTE FUR CHEMIE, cilt.149, sa.9, ss.1535-1542, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 149 Sayı: 9
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s00706-018-2223-x
  • Dergi Adı: MONATSHEFTE FUR CHEMIE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1535-1542
  • Anahtar Kelimeler: High-performance liquid chromatography, Decomposition, Long-term stability, Mass spectrometry, Emetine, Cephaeline, PERFORMANCE LIQUID-CHROMATOGRAPHY, EMETINE, CEPHAELINE, BIOSYNTHESIS, SYRUP, ASSAY, ROOT
  • Ankara Üniversitesi Adresli: Evet

Özet

The alkaloids in two historical pharmaceutical relics of ipecacuanha, dated to the eighteenth century, were analyzed by HPLC-MS. The conditions of extraction and separation were optimized. Four alkaloids (emetine, cephaeline, protoemetine, and O-methylpsychotrine) were found. The content of the main ipecacuanha alkaloids, emetine and cephaeline, in analyzed samples is fairly decreased in comparison with standard current pharmaceutical substance. O-Methylpsychotrine has been identified as the emetine decomposition product. The identity of the compounds was confirmed by MS2, and the ESI+ fragmentation mechanisms of compounds found have been proposed.