HPLC-MS analysis of ipecacuanha alkaloids in pharmaceutical relics from eighteenth century
MONATSHEFTE FUR CHEMIE, cilt.149, sa.9, ss.1535-1542, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 149 Sayı: 9
- Basım Tarihi: 2018
- Doi Numarası: 10.1007/s00706-018-2223-x
- Dergi Adı: MONATSHEFTE FUR CHEMIE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1535-1542
- Anahtar Kelimeler: High-performance liquid chromatography, Decomposition, Long-term stability, Mass spectrometry, Emetine, Cephaeline, PERFORMANCE LIQUID-CHROMATOGRAPHY, EMETINE, CEPHAELINE, BIOSYNTHESIS, SYRUP, ASSAY, ROOT
- Ankara Üniversitesi Adresli: Evet
Özet
The alkaloids in two historical pharmaceutical relics of ipecacuanha, dated to the eighteenth century, were analyzed by HPLC-MS. The conditions of extraction and separation were optimized. Four alkaloids (emetine, cephaeline, protoemetine, and O-methylpsychotrine) were found. The content of the main ipecacuanha alkaloids, emetine and cephaeline, in analyzed samples is fairly decreased in comparison with standard current pharmaceutical substance. O-Methylpsychotrine has been identified as the emetine decomposition product. The identity of the compounds was confirmed by MS2, and the ESI+ fragmentation mechanisms of compounds found have been proposed.