Effect of 7-dehydrocholesterol and Cholesterol-loaded Cyclodextrins on Bull Sperm Motility During Short Term Storage

İNANÇ M. E., TEKİN K., OLĞAÇ K. T., ÖZEN D., Stelletta C., UYSAL O., ...Daha Fazla

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.23, sa.4, ss.661-664, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 4
  • Basım Tarihi: 2017
  • Doi Numarası: 10.9775/kvfd.2017.17181
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.661-664
  • Anahtar Kelimeler: Bull sperm, Cholesterol, Motility, Short term storage, 7-dehydrocholesterol, LECITHIN-BASED EXTENDER, CRYOSURVIVAL, QUALITY
  • Ankara Üniversitesi Adresli: Evet

Özet

The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4 degrees C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4 degrees C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x10(6) group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4 degrees C.