Effect of 7-dehydrocholesterol and Cholesterol-loaded Cyclodextrins on Bull Sperm Motility During Short Term Storage
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.23, sa.4, ss.661-664, 2017 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 23 Sayı: 4
- Basım Tarihi: 2017
- Doi Numarası: 10.9775/kvfd.2017.17181
- Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.661-664
- Anahtar Kelimeler: Bull sperm, Cholesterol, Motility, Short term storage, 7-dehydrocholesterol, LECITHIN-BASED EXTENDER, CRYOSURVIVAL, QUALITY
- Ankara Üniversitesi Adresli: Evet
Özet
The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4 degrees C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4 degrees C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x10(6) group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4 degrees C.