Akademik Veri Yönetim Sistemi
JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.13, sa.3, ss.285-292, 2007 (SCI-Expanded)
Some common wheat varieties such as Temmuz 2, Iraq, Eliz 66, Iba 99 Musaddak, Iba 99 Museccel which are important as genetic material and in agriculture of Iraq were used in this study. Experiment was carried out at the biotechnology laboratories of Department of Field Crops, Agriculture Faculty of Ankara University; the aim of this research was to determine the callus induction and plant regeneration for these varieties under in vitro conditions. For this purpose 8 ml/l 2,4-D was used for callus induction and 20 g/l agar was used for plant regeneration as MS. Parameters such as callus induction, callus weight, regeneration capacity, culture efficiency and number of regenerated plant were measured in this study. According to obtained result; callus induction was % 88.75 (Eliz 66) - % 98.75 (Iba 99 Musaddak), callus weight was 0.7025 g (Temmuz 2) - 7.2179 g (Eliz 66), regeneration capacity was % 86.75 (Eliz 66) - % 98. 99 Musaddak), cluture efficiency was % 77.50 (Eliz 66) - % 97.50 (Iba 99 Musaddak) and number of regenerated plants was 3.500 (Iba 99 Musaddak) - 16.26 (Temmuz 2). In addition, when investigated relationships among traits; there was significant relationship between callus weight and number of regenerated plants (r = 0.674**). And also, regeneration capacity was effected as significantly by callus weight (r = 0.900**). Callus induction and regeneration capacity or their potential of the production process of trans genetic plant were investigated of some Iraqi common wheat varieties (which are resistant / tolarent to the drought, salt and some abiotic stress factors) in this research; and also, it was determined Irak variety is most favorable for this kind of studies.