Heavy metal accumulation and genotoxicity indicator capacity of the lichen species Ramalina pollinaria collected from around an iron steel factory in Karabuk, Turkey

CANSARAN DUMAN D., Altunkaynak E., ARAS E. S.

TURKISH JOURNAL OF BOTANY, cilt.38, sa.3, ss.477-490, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 3
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3906/bot-1201-19
  • Dergi Adı: TURKISH JOURNAL OF BOTANY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.477-490
  • Anahtar Kelimeler: Air pollution, lichen, RAPD, genotoxicity, AMPLIFIED POLYMORPHIC DNA, AIR-POLLUTION, PROVINCE TURKEY, DAMAGE, RAPD, CADMIUM, PLANTS, L., DETECT, CARCINOGENESIS
  • Ankara Üniversitesi Adresli: Evet

Özet

In the present work, Ramalina pollinaria (Westr.) Ach. specimens collected from around an iron steel factory in Karabuk were analyzed due to their heavy metal accumulation by atomic absorption spectrometry. The specimens were also evaluated for pollution-induced DNA damage by random amplified polymorphic DNA (RAPD) assay. Genomic template stabilities were calculated from the changes in RAPD profiles and compared with heavy metal content in R. pollinaria. The results obtained from chemical analysis suggest that R. pollinaria is a particularly suitable lichen species for the detection of air quality. Results of RAPD assay showed significant differences in band patterns in R. pollinaria as compared to the control sample with respect to disappearance and appearance of bands. In this study, we have assessed the potential of RAPD assay as an application tool for detecting the genotoxic effect of air pollutants according to the changes in DNA band patterns in R. pollinaria.