Akademik Veri Yönetim Sistemi
Plant Biosystems, cilt.160, sa.3, 2026 (SCI-Expanded, Scopus)
Genista species, belonging to the Fabaceae (Leguminosae) family, comprise 16 taxa, six of which are endemic to Türkiye. This study is the first to investigate the in vitro cytotoxic, anti-inflammatory, and antioxidant activities of the aerial parts of the endemic Genista burdurensis. The methanolic extract was fractionated into four phases of varying polarities (n-hexane, chloroform, ethyl acetate, and aqueous) using liquid–liquid extraction. The in vitro cytotoxic, anti-inflammatory, and antioxidant activities of these phases were also evaluated. The ethyl acetate phase, which exhibited the highest activity, was selected for further isolation. Through chromatographic separation methods, two isoflavones and four flavonoid compounds were isolated. Among the isolated compounds, the five major constituents (apigenin-7-O-β-glucoside, genistein-7-O-β-glucoside, genistein-8-C-glucoside, luteolin, and luteolin-7-O-β-glucoside) were analyzed quantitatively by reverse-phase HPLC method. In conclusion, luteolin-7-O-β-glucoside was found to have the strongest cytotoxic activity against HL60 (human promyelocytic leukemia) and HCT-116 (human colon cancer) cell lines, while genistein-7-O-β-glucoside showed cytotoxic effect in HCT-116 cells but not in HL60 cells. The ethyl acetate phase and isolated flavonoids displayed significant anti-inflammatory activity, as evidenced by the substantial nitrite inhibition in LPS/IFNγ-stimulated RAW 264.7 macrophage cells. Furthermore, luteolin-7-O-β-glucoside was identified as the most potent antioxidant compound based on its radical-scavenging activity in antioxidant assays.