Identity and pathogenicity of Fusarium species associated with crown rot on wheat (Triticum spp.) in Turkey

Gebremariam E. S., Sharma-Poudyal D., Paulitz T. C., Erginbas-Orakci G., Karakaya A., Dababat A. A.

EUROPEAN JOURNAL OF PLANT PATHOLOGY, cilt.150, sa.2, ss.387-399, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 150 Sayı: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s10658-017-1285-7
  • Dergi Adı: EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.387-399
  • Anahtar Kelimeler: Crown rot, Fusarium, Translation elongation factor, Pathogenicity, Virulence, Bread wheat, Triticum aestivum, Durum wheat, Triticum durum, GRASS STEM BASES, NEW-SOUTH-WALES, WINTER-WHEAT, ROOT-ROT, 1ST REPORT, MOLECULAR-IDENTIFICATION, PACIFIC-NORTHWEST, GENETIC DIVERSITY, GROWTH-STAGES, SPRING WHEAT
  • Ankara Üniversitesi Adresli: Evet

Özet

An extensive survey was carried out to collect Fusarium species colonizing the lower stems (crowns) of bread wheat (Triticum aestivum L.) and durum wheat (T. durum Desf.) from different wheat growing regions of Turkey in summer 2013. Samples were collected from 200 fields representing the major wheat cultivation areas in Turkey, and fungi were isolated from symptomatic crowns. The isolates were identified to species level by sequencing the translation elongation factor 1-alpha (TEF1-alpha) gene region using primers ef1 and ef2. A total of 339 isolates representing 17 Fusarium species were isolated. The isolates were identified as F. culmorum, F. pseudograminearum, F. graminearum, F. equiseti, F. acuminatum, F. brachygibbosum, F. hostae, F. redolens, F. avenaceum, F. oxysporum, F. torulosum, F. proliferatum, F. flocciferum, F. solani, F. incarnatum, F. tricinctum and F. reticulatum. Fusarium equiseti was the most commonly isolated species, accounting for 36% of the total Fusarium species isolated. Among the damaging species, F. culmorum was the predominant species being isolated from 13.6% of sites surveyed while F. pseudograminearum and F. graminearum were isolated only from 1% and 0.5% of surveyed sites, respectively. Six out of the 17 Fusarium species tested for pathogenicity caused crown rot with different levels of severity. Fusarium culmorum, F. pseudograminearum and F. graminearum caused severe crown rot disease on durum wheat. Fusarium avenaceum and F. hostae were weakly to moderately virulent. Fusarium redolens was weakly virulent. However, F. oxysporum, F. equiseti, F. solani, F. incarnatum, F. reticulatum, F. flocciferum, F. tricinctum, F. brachygibbosum, F. torulosum, F. acuminatum and F. proliferatum were non-pathogenic. The result of this study reveal the existence of a wide range of Fusarium species associated with crown rot of wheat in Turkey.