Disposable Bienzymatic Choline Biosensor Based on MnO(2)Nanoparticles Decorated Carbon Nanofibers and Poly(methylene green) Modified Screen Printed Carbon Electrode

KAÇAR SELVİ C.

ELECTROANALYSIS, cilt.32, sa.10, ss.2118-2127, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 10
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/elan.202060010
  • Dergi Adı: ELECTROANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2118-2127
  • Anahtar Kelimeler: Amperometric biosensor, Choline, Carbon nanofiber, Manganese dioxide nanoparticles, Poly(methylene green), MNO2 NANOPARTICLES, HORSERADISH-PEROXIDASE, HYDROGEN-PEROXIDE, GRAPHENE OXIDE, PERFORMANCE, IMMOBILIZATION, COMPOSITES, NANOTUBES, SENSOR
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, utilization of MnO(2)nanoparticles decorated carbon nanofibers (CNF-MnO2) and poly(methylene green) (PMG) to create a bienzymatic biosensor for choline determination is described. Choline oxidase (ChOx) and horseradish peroxidase (HRP) were co-immobilized onto the CNF-MnO2/PMG/SPCE by glutaraldehyde crosslinking. Scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to investigate the morphology and electrochemical behavior of the modified electrodes. Upon optimized conditions, ChOx-HRP/CNF-MnO2/PMG/SPCE had a wide working range of 4.0x10(-6)-9.0x10(-3) M with a sensitivity of 2.44 mu A mM(-1)and detection limit of 8.1x10(-7) M. The proposed biosensor exhibits high repeatability (RSD 2.7 %) and retained 97 % of its initial activity after stored 15 days at +4 degrees C. The applicability of the biosensor in infant formula was demonstrated.