Electrochemical study of S-adenosyl-L-methionine and its differential pulse and square-wave voltammetric determination

Uslu B., Ozkan S., Aboul-Enein H.

ELECTROANALYSIS, cilt.14, sa.11, ss.736-740, 2002 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 11
  • Basım Tarihi: 2002
  • Dergi Adı: ELECTROANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.736-740
  • Anahtar Kelimeler: S-adenosyl-L-methionine (SAM), differential pulse voltammetry, square-wave voltammetry, pharmaceutical preparations, LIQUID-CHROMATOGRAPHIC PROCEDURE, NATURAL POLYAMINES, ADENOSYLMETHIONINE, OXIDATION, PHARMACEUTICALS, TABLETS, METABOLITES, DISORDERS, BEHAVIOR, TISSUES
  • Ankara Üniversitesi Adresli: Evet

Özet

The electrochemical oxidation of S-adenosyl-L-methionine (SAM) has been carried out in aqueous solution in the pH range 1.5 - 11.0 by cyclic, linear sweep, differential pulse (DPV) and square-wave (SWV)voltammetry. SAM exhibits one oxidation peak or wave depending on pH. The oxidation process was shown to be irreversible. For analytical purposes, a very well resolved diffusion controlled voltammetric peak was obtained in phosphate buffer at pH 2.04 for DPV and SWV techniques. The linear response was obtained in the ranges of 2 x 10(-5)-6 x 10(-4) M for both techniques with a detection limit of 2.6 x 10(-6) M for DPV and 2.4 x 10(-4) M for SWV Based on this study, simple. rapid and selective two voltammetric methods were developed for the determination of SAM in tablet dosage forms.